KHOOM PLIG

Lactobacillus kab mobyog ib qho ntawm ntau hom kab mob uas muaj nyob hauv genus Lactobacillus. Nws yog cov kab mob mesophilic uas yog gram zoo, zoo li tus pas nrig, nonsporing, nonmotile, anaerobic, thiab tsis muaj cytochromes.L. castuaj yeem pom nyob rau hauv ntau qhov chaw xws li cov khoom siv mis nyuj nyoos thiab fermented, plab hnyuv thiab cov kab mob ntawm cov tib neeg thiab tsiaj txhu, thiab cov khoom cog tshiab thiab fermented. Qhov zoo tshaj pH rauL. casyog 5.5. Cov lactic acid tsim los ntawmL. caslos ntawm fermentation yog ib qho tseem ceeb heev vim nws tuaj yeem siv los ua cheese thiab yogurts, txo cov roj cholesterol, txhim khu kev tiv thaiv kab mob, tswj raws plab, txo lactose intolerance, inhibit plab hnyuv kab mob, thiab ua hauj lwm raws li probiotics. Probiotics yog cov kab mob uas siv tau los txhawb lossis txhawb kev sib npaug ntawm cov microbes kom nyob hauv txoj hnyuv.

TSEEM CEEB

Strain npe	Lactobacilluscasi	Lim tus lej	PB-LC39
Muaj peev xwm	300 bn cfu/g	Gene Identification	Yog lawm
Tso nyiaj	CCTCC	Ntawv pov thawj	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Ntim	500g, 1kg lossis 5kg rau ib lub hnab ntawv ci	Cia	-18 degree rau lub sijhawm ntev, 2-8 degree rau lub sijhawm luv

TECHNICAL Indicators

Cov khoom		Specification
1) Nyob Lactobacillus kab mob hwj chim		Ntau dua lossis sib npaug rau 3.0 × 10¹¹CFU/g
2) Cov ntsiab lus dej		Tsawg dua lossis sib npaug rau 8.0 feem pua
4) Cov coliforms		Tsawg dua lossis sib npaug li 10CFU / g
5) Moulds, Poov xab		Tsawg dua lossis sib npaug li 10CFU / g
6) Cov kab mob pathogenic		Tsis zoo
7) Cov hlau hnyav	Lead (Pb)	Tsawg dua lossis sib npaug li 1ppm
	Arsenic (As)	Tsawg dua lossis sib npaug rau 0.5ppm

TSEEM CEEB

Hom Lub Npe

Kev txheeb xyuas

Deposit No.

Antibiotic Susceptibility

Acid thiab Bile ntsev tsis kam

Kev ruaj ntseg

Qhov System

L. casPB-LC39

16 S rRNA

CCTCC NO: M2018886

Ua tiav

SC/ISO9001/ISO22000/FSSC22000/HACCP/

HALAL/KOSHER/FDA

ACID TOLERANCE

Acid resistance ntawmLactobacillus kab mobPB-LC39 hmoov tau ua tiav ntawm pH =2 0 thiab pH =3 0 mus txog 4 teev. Lub microbial enumeration ntawmLactobacillus kab mobPB-LC39 yuav tsum tau ua tsis ntau tshaj li qhov pib (raws li kev tswj), 1 teev, 2 teev, thiab 4 teev lub sij hawm cov ntsiab lus. Daim duab 1 qhia txog qhov ntawdLactobacillus kab mobPB-LC39 tuaj yeem muaj sia nyob ntawm pH qis, uas txhais tau hais tias cov kab no muaj cov kua qaub zoo.

Fig 1. Acid kuj ntawmL. casPB-LC39

BILE SALT TOLERANCE

Bile ntsev kuj ntawmLactobacillus kab mobPB-LC39 hmoov tau ua tiav ntawm 0.2 feem pua thiab 0.3 feem pua cov kua tsib ntsev mus txog 4 teev. Lub microbial enumeration ntawmLactobacillus kab mobPB-LC39 yuav tsum tau ua tsis ntau tshaj li qhov pib (raws li kev tswj), 1 teev, 2 teev, thiab 4 teev lub sij hawm cov ntsiab lus. Daim duab 2 qhia txog qhov ntawdLactobacillus kab mobPB-LC39 tuaj yeem muaj sia nyob ntawm qhov siab ntawm cov kua tsib ntsev, uas txhais tau hais tias cov kab mob no muaj cov kua tsib ntsev zoo.

Fig 2. Bile ntsev kuj ntawmL. casPB-LC39

KHOOM PLIG

Lactobacillus kab mobPB-LC39 hmoov tau muab ntim rau hauv lub hnab aluminium ntawv ci, khaws cia ntawm -18 degree rau 24 lub hlis rau kev ntsuas kev ruaj ntseg mus sij hawm ntev. Lub microbial enumeration ntawmLactobacillus kab mobPB-LC39 yuav tsum tau ua tsis ntau ntau tshaj li qhov pib, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 lub hlis lub sij hawm cov ntsiab lus. Raws li daim duab 3, lub potency ntawmLactobacillus kab mobPB-LC39 hmoov yog 2.87 × 10¹¹CFU/g. Tom qab lub sijhawm cia ntawm 24 lub hlis, lub zog ntawm cov hmoov yog 2.18 × 10¹¹CFU/g. Nws txhais tau tias cov kab mob no tuaj yeem nyob ruaj khov rau lub sijhawm ntev yog tias khaws cia ntawm qhov kub thiab txias.

Fig 3. Cia stability ntawmL. casPB-LC39

KEV PAB CUAM

1. Immunomodulation

Natural killer (NK) hlwb yog cov lymphocytes ntawm lub cev tiv thaiv kab mob. Cov hlwb no ua lub luag haujlwm tseem ceeb hauv kev tiv thaiv kab mob thiab hauv kev tiv thaiv kab mob ntawm cov qog.Tom qab 30-hnub intragastric kev tswj hwm ntawmL. casPB-LC39 hauv nas, cov txiaj ntsig (Daim duab 4.) tau pom tiasL. casPB-LC39 nce cytotoxicity ntawm NK hlwb nyob rau hauv ib koob tshuaj.

Fig 4.L. casPB-LC39 cov teebmeem ntawm cytotoxicity ntawm NK hlwb

2. Txo cov oxidative kev nyuaj siab hauv daim siab

Oxidative stress yog ib qho tshwm sim los ntawm qhov tsis txaus ntseeg ntawm kev tsim khoom thiab sib sau ntawm cov pa oxygen reactive hom (ROS) hauv cov hlwb thiab cov ntaub so ntswg thiab lub peev xwm ntawm cov kab mob lom los detoxify cov khoom reactive. Oxidative stress indicators suav nrog superoxide dismutase (SOD) thiab malondialdehyde (MDA) tau sim hauv cov tsiaj sim. Cov txiaj ntsig (Table 2) tau pom tias cawv tuaj yeem ua rau muaj kev ntxhov siab oxidative hauv daim siab thiab tom qabL. casei PB-LC39kev kho mob, SOD thiab MDA rov qab mus rau theem nomal, uas txhais tau tiasL. casPB-LC39 (Table 1.) tuaj yeem txo oxidative kev nyuaj siab hauv daim siab.

Oxidative stress indicators	SOD, U/mgprot	MDA, U/mgprot
Tswj pawg	48.82±2.96	0.75±0.05
Kev kho dej cawv (mM)	1.0	32.74±1.31	1.33±0.10
L. casPB-LC39 kev kho mob (cfu/mL)	1.0×10⁵	31.69±0.95	1.39±0.19
	1.0×10⁶	36.78±0.77	1.06±0.12
	1.0×10⁷	42.14±1.49	0.99±0.02
	1.5×10⁷	42.34±2.12	0.98±0.06

Rooj 1. Cov teebmeem ntawmL. casPB-LC39 kev kho mob hauv siab oxidative kev nyuaj siab

3. Txo cov roj cholesterol

Tag nrho cov roj (cholesterol) yog tag nrho cov roj (cholesterol) hauv cov ntshav. Hauv kev sim, peb pom qhov ntawdL. casPB-LC39 (Table 2.) kev kho mob tuaj yeem txo cov roj cholesterol tag nrho piv rau qib pib thiab kev kho cov placebo.

	Pib		6 lis		12 lub lis piam
	Cov tshuaj placebo	L. cas	Cov tshuaj placebo	L. cas	Cov tshuaj placebo	L. cas
>2500 mg/l TC (mg / l)	2703	2769	2550	2555	2574	2286

<2500> TC (mg / l)	2295	2217	2189	2131	2219	2008