KHOOM PLIG

Bifidobacterium longumHH-BL18 yog cov kab mob Gram-positive, catalase-negative, rod-zoo li tus kab mob tam sim no nyob rau hauv tib neeg txoj hnyuv thiab ib qho ntawm 32 hom uas muaj nyob rau hauv genus Bifidobacterium. Nws yog ib qho microaerotolerant anaerobe thiab suav tias yog ib qho ntawm cov kab mob ntxov tshaj plaws ntawm txoj hnyuv ntawm cov me nyuam mos. Thaum loj hlob ntawm qhov nruab nrab anaerobic,B. longumdaim ntawv dawb, glossy colonies nrog ib tug convex zoo.B. longumyog non-pathogenic thiab feem ntau ntxiv rau cov khoom noj.

TSEEM CEEB

Strain npe	Bifidobacteriumntev	Lim tus lej	HH-BL18
Muaj peev xwm	100bn cfu/g	Gene Identification	Yog lawm
Tso nyiaj	CCTCC	Ntawv pov thawj	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Ntim	500g, 1kg lossis 5kg rau ib lub hnab ntawv ci	Cia	-18 degree rau lub sijhawm ntev, 2-8 degree rau lub sijhawm luv

TECHNICAL Indicators

Cov khoom		Specification
1) NyobBifidobacteriumntev hwj chim		Ntau dua lossis sib npaug rau 1.0 × 10¹¹CFU/g
2) Cov ntsiab lus dej		Tsawg dua lossis sib npaug rau 8.0 feem pua
4) Cov coliforms		Tsawg dua lossis sib npaug li 10CFU / g
5) Moulds, Poov xab		Tsawg dua lossis sib npaug li 10CFU / g
6) Cov kab mob pathogenic		Tsis zoo
7) Cov hlau hnyav	Lead (Pb)	Tsawg dua lossis sib npaug li 1ppm
	Arsenic (As)	Tsawg dua lossis sib npaug rau 0.5ppm

TSEEM CEEB

Hom Lub Npe

Kev txheeb xyuas

Deposit No.

Antibiotic Susceptibility

Acid thiab Bile ntsev tsis kam

Kev ruaj ntseg

Qhov System

B.ntevHH-BL18

16 S rRNA

Ua tiav

SC/ISO9001/ISO22000/FSSC22000/

HACCP/HALAL/

KOSHER/FDA

ACID TOLERANCE

Acid resistance ntawmBifidobacterium longumHH-BL18 hmoov tau ua los ntawm pH =2 0 thiab pH =3 0 mus txog 4 teev. Lub microbial enumeration ntawmBifidobacterium longumHH-BL18 yuav tsum tau ua tsis pub ntau tshaj li qhov pib (raws li kev tswj), 1 teev, 2 teev, thiab 4 teev lub sij hawm cov ntsiab lus. Daim duab 1 qhia txog qhov ntawdBifidobacterium longumHH-BL18 tuaj yeem muaj sia nyob ntawm qis pH, uas txhais tau hais tias cov kab no muaj cov kua qaub zoo.

Fig 1. Acid kuj ntawmB.longumHH-BL18

BILE SALT TOLERANCE

Bile ntsev kuj ntawmBifidobacterium longumHH-BL18 hmoov tau ua tiav ntawm 0.2 feem pua thiab 0.3 feem pua ntawm cov kua tsib ntsev mus txog 4 teev. Lub microbial enumeration ntawmBifidobacterium longumHH-BL18 yuav tsum tau ua tsis pub ntau tshaj li qhov pib (raws li kev tswj), 1 teev, 2 teev, thiab 4 teev lub sij hawm cov ntsiab lus. Daim duab 2 qhia txog qhov ntawdBifidobacterium longumHH-BL18 tuaj yeem muaj sia nyob ntawm qhov siab ntawm cov kua tsib ntsev, uas txhais tau tias cov kab mob no muaj cov kua tsib ntsev ua haujlwm zoo.

Fig 2. Bile ntsev kuj ntawmB.longumHH-BL18

KHOOM PLIG

Bifidobacterium longumHH-BL18 hmoov tau muab ntim rau hauv lub hnab aluminium ntawv ci, khaws cia ntawm -18 degree rau 24 lub hlis rau kev ntsuas kev ruaj ntseg mus sij hawm ntev. Lub microbial enumeration ntawmBifidobacterium longumHH-BL18 yuav tsum tau ua tsis pub ntau tshaj li qhov pib, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 lub hlis lub sij hawm cov ntsiab lus. Raws li daim duab 3, lub potency ntawmBifidobacterium longumHH-BL18 hmoov yog 1.51 × 10¹¹CFU/g. Tom qab lub sijhawm cia ntawm 24 lub hlis, lub zog ntawm cov hmoov yog 1.15 × 10¹¹CFU/g. Nws txhais tau tias cov kab mob no tuaj yeem nyob ruaj khov rau lub sijhawm ntev yog tias khaws cia ntawm qhov kub thiab txias.

Fig 3. Cia stability ntawmB.longumHH-BL18

KEV PAB CUAM

1. Kho cem quav

Short-chain fatty acids (SCFAs) yog ib feem ntawm fatty acids uas yog tsim los ntawm lub plab microbiota thaum lub sij hawm fermentation ntawm ib nrab thiab nondigestible polysaccharides. SCFAs tuaj yeem ua rau muaj kev sib daj sib deev, ua rau kom nrawm nrawm thiab tshem tawm cem quav. Hauv kev sim (Daim duab 4), peb pom tias qib SCFAs tau ntau dua hauv cov qauv fecal sau los ntawm cov neeg uas muaj kev kho mob probiotic (Bifidobacterium longumHH-BL18).

Fig 4.B.longumHH-BL18 tuaj yeem txo qhov cem quav los ntawm kev nce qib SCFAs.

2. Ntsuas lub plab microbiota

Hauv kev sim tib neeg (Daim duab 5), peb pom tias qhov ncauj noj ntawmB.longumHH-BL18 tuaj yeem txhim kho cov kab mob microbial hauv plab dysbiosis los ntawm kev nce qib ntawm cov kab mob zoo bacteira thiab txo qis cov kab mob phem.