KHOOM PLIG

Bifidobacterium lactis HH-BA68 hmoov muaj cov Bifidobacterium lactis HH-BA68 thiab tus neeg saib xyuas tshwj xeeb. Cov kab mob no tau raug cais tawm los ntawm cov menyuam mos noj qab nyob zoo, nws tuaj yeem muaj sia nyob hauv thaj chaw acidic gastric thiab ncav cuag cov hnyuv me thiab colonize tus tswv tsev. Hauv qhov twg, nws ua ntau txoj hauv kev los txhim kho kev noj qab haus huv.

TSEEM CEEB

Strain npe	Bifidobacterium lactis	Lim tus lej	HH-BA68
Muaj peev xwm	500 bn cfu/g	Gene Identification	Yog lawm
Tso nyiaj	CCTCC	Ntawv pov thawj	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Ntim	500g, 1kg lossis 5kg rau ib lub hnab ntawv ci	Cia	-18 degree rau lub sijhawm ntev, 2-8 degree rau lub sijhawm luv

TECHNICAL Indicators

Cov khoom		Specification
1) NyobBifidobacterium lactishwj chim		Ntau dua lossis sib npaug rau 5.0 × 1011 CFU/g
2) Cov ntsiab lus dej		Tsawg dua lossis sib npaug rau 8.0 feem pua
4) Cov coliforms		Tsawg dua lossis sib npaug li 10CFU / g
5) Moulds, Poov xab		Tsawg dua lossis sib npaug li 10CFU / g
6) Cov kab mob pathogenic		Tsis zoo
7) Cov hlau hnyav	Lead (Pb)	Tsawg dua lossis sib npaug li 1ppm
	Arsenic (As)	Tsawg dua lossis sib npaug rau 0.5ppm

TSEEM CEEB

Hom Lub Npe

Kev txheeb xyuas

Deposit No.

Antibiotic Susceptibility

Acid thiab Bile ntsev tsis kam

Kev ruaj ntseg

Qhov System

B.lactisHH-BA68

16 S rRNA

Ua tiav

SC/ISO9001/ISO22000/FSSC22000/HACCP/

HALAL/KOSHER/FDA

ACID TOLERANCE

Acid resistance ntawmBifidobacterium lactisHH-BA68 hmoov tau ua los ntawm pH =2 0 thiab pH =3 0 mus txog 4 teev. Lub microbial enumeration ntawmBifidobacterium lactisHH-BA68 yuav tsum tau ua tsis ntau tshaj li qhov pib (raws li kev tswj), 1 teev, 2 teev, thiab 4 teev lub sij hawm cov ntsiab lus. Daim duab 1 qhia txog qhov ntawdBifidobacterium lactisHH-BA68 tuaj yeem muaj sia nyob ntawm qis pH, uas txhais tau hais tias cov kab no muaj cov kua qaub zoo.

Fig 1. Acid kuj ntawmB.lactisHH-BA68

BILE SALT TOLERANCE

Bile ntsev kuj ntawmBifidobacterium lactisHH-BA68 hmoov tau ua tiav ntawm 0.2 feem pua thiab 0.3 feem pua cov kua tsib ntsev mus txog 4 teev. Lub microbial enumeration ntawmBifidobacterium lactisHH-BA68 yuav tsum tau ua tsis ntau tshaj li qhov pib (raws li kev tswj), 1 teev, 2 teev, thiab 4 teev lub sij hawm cov ntsiab lus. Daim duab 2 qhia txog qhov ntawdBifidobacterium lactisHH-BA68 tuaj yeem muaj sia nyob ntawm qhov siab ntawm cov kua tsib ntsev, uas txhais tau hais tias cov kab mob no muaj cov kua tsib ntsev zoo.

Fig 2. Bile ntsev kuj ntawmB.lactis HH-BA68

KHOOM PLIG

Bifidobacterium lactisHH-BA68 hmoov tau muab ntim rau hauv lub hnab aluminium ntawv ci, khaws cia ntawm -18 degree rau 24 lub hlis rau kev ntsuas kev ruaj ntseg mus sij hawm ntev. Lub microbial enumeration ntawmBifidobacterium lactisHH-BA68 yuav tsum tau ua tsis ntau ntau tshaj li qhov pib, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 lub hlis cov ntsiab lus. Raws li daim duab 3, lub potency ntawmBifidobacterium lactisHH-BA68 hmoov yog 4.40 × 10¹¹CFU/g. Tom qab lub sijhawm cia ntawm 24 lub hlis, lub zog ntawm cov hmoov yog 3.60 × 10¹¹CFU/g. Nws txhais tau tias cov kab mob no tuaj yeem nyob ruaj khov rau lub sijhawm ntev yog tias khaws cia ntawm qhov kub thiab txias.

Fig 3. Cia stability ntawmB.lactisHH-BA68

KEV PAB CUAM

1.Inhibit pathogen colonization

Lub hom phiaj ntawm txoj kev tshawb no yog kev ntsuam xyuas ntawm antagonistic nyhuv ntawmB.lactisHH-BA68 tiv thaiv colonization los ntawm cov kab mob. Cov txiaj ntsig tau pom (Table 1) ntawdB.lactisHH-BA68 yog qhov tseem ceeb hauv kev muab kev tiv thaiv tiv thaiv kab mob los ntawm plaub kab mob kab mob.

	S.aureus	C. nyuaj	S. typhimurium	L.monocytogenes
Colonization inhibition tus nqi (%)	48.0±3.6	27.8±6.4	5.2±3.8	25.7±8.0
Colonization hloov tus nqi (%)	25.7±7.5	56.7±7.4	7.4±2.8	43.8±7.3

Rooj 1. Pothogen colonization inhibitory muaj peev xwm ntawmB.lactisHH-BA68

2.Inhibit pathogen loj hlob

Lub hom phiaj ntawm txoj kev tshawb no yog kev ntsuam xyuas ntawm antagonistic nyhuv ntawmB.lactisHH-BA68 tiv thaiv plaub kab mob. Antimicrobial teebmeem ntawm kev sib cais tau soj ntsuam los ntawm kev kuaj disc diffusion ntawm daim hlau nrog cov kab mob sib txawv. Kev loj hlob inhibition cheeb tsam ntawm cov kab mob thiabB.lactisHH-BA68 inhibitory muaj peev xwm raug soj ntsuam tom qab incubation ntawm tag nrho cov agar media ntawm 37 degree rau 24h. Cov txiaj ntsig tau pom (Table 2) tiasB.lactisHH-BA68 had relatively strong activity against tested pathogens (with inhibition zone diameter>15 hli).