KHOOM PLIG

BifidobacteriumbreveHH-BR26 yog ib hom kab mob ntawm cov genus Bifidobacterium uas muaj cov khoom probiotic. Nws yog cov kab mob uas muaj txiaj ntsig pom nyob rau hauv tib neeg lub cev hauv plab hnyuv ntawm cov menyuam mos thiab cov neeg laus, nrog rau hauv cov kua mis.

TSEEM CEEB

Strain npe	Bifidobacteriumbreve	Lim tus lej	HH-BR26
Muaj peev xwm	300 bn cfu/g	Gene Identification	Yog lawm
Tso nyiaj	CCTCC	Ntawv pov thawj	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Ntim	500g, 1kg lossis 5kg rau ib lub hnab ntawv ci	Cia	-18 degree rau lub sijhawm ntev, 2-8 degree rau lub sijhawm luv

TECHNICAL Indicators

Cov khoom		Specification
1) NyobBifidobacteriumbreve hwj chim		Ntau dua lossis sib npaug rau 3.0 × 10¹¹CFU/g
2) Cov ntsiab lus dej		Tsawg dua lossis sib npaug rau 8.0 feem pua
4) Cov coliforms		Tsawg dua lossis sib npaug li 10CFU / g
5) Moulds, Poov xab		Tsawg dua lossis sib npaug li 10CFU / g
6) Cov kab mob pathogenic		Tsis zoo
7) Cov hlau hnyav	Lead (Pb)	Tsawg dua lossis sib npaug li 1ppm
	Arsenic (As)	Tsawg dua lossis sib npaug rau 0.5ppm

TSEEM CEEB

Hom Lub Npe

Kev txheeb xyuas

Deposit No.

Antibiotic Susceptibility

Acid thiab Bile ntsev tsis kam

Kev ruaj ntseg

Qhov System

B.longumHH-BL18

16 S rRNA

Ua tiav

SC/ISO9001/ISO22000/FSSC22000/

HACCP/HALAL/

KOSHER/FDA

ACID TOLERANCE

Acid resistance ntawmBifidobacterium breveHH-BR26 hmoov tau ua tiav ntawm pH =2 0 thiab pH =3 0 mus txog 4 teev. Lub microbial enumeration ntawmBifidobacterium breveHH-BR26 yuav tsum tau ua tsis ntau tshaj li qhov pib (raws li kev tswj), 1 teev, 2 teev, thiab 4 teev lub sij hawm cov ntsiab lus. Daim duab 1 qhia txog qhov ntawdBifidobacterium breveHH-BR26 tuaj yeem muaj sia nyob ntawm pH qis, uas txhais tau hais tias cov kab no muaj cov kua qaub zoo.

Fig 1. Acid kuj ntawmB.breveHH-BR26

BILE SALT TOLERANCE

Bile ntsev kuj ntawmBifidobacterium breveHH-BR26 hmoov tau ua tiav ntawm 0.2 feem pua thiab 0.3 feem pua ntawm cov kua tsib ntsev mus txog 4 teev. Lub microbial enumeration ntawmBifidobacterium breveHH-BR26 yuav tsum tau ua tsis ntau tshaj li qhov pib (raws li kev tswj), 1 teev, 2 teev, thiab 4 teev lub sij hawm cov ntsiab lus. Daim duab 2 qhia txog qhov ntawdBifidobacterium breveHH-BR26 tuaj yeem muaj sia nyob ntawm qhov siab ntawm cov kua tsib ntsev, uas txhais tau hais tias cov kab mob no muaj cov kua tsib ntsev zoo.

Fig 2. Bile ntsev kuj ntawmB.breveHH-BR26

KHOOM PLIG

Bifidobacterium breveHH-BR26 hmoov tau muab ntim rau hauv lub hnab aluminium ntawv ci, khaws cia ntawm -18 degree rau 24 lub hlis rau kev ntsuas kev ruaj ntseg mus sij hawm ntev. Lub microbial enumeration ntawmBifidobacterium breveHH-BR26 yuav tsum tau ua tsis pub ntau tshaj li qhov pib, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 lub hlis lub sij hawm cov ntsiab lus. Raws li daim duab 3, lub potency ntawmBifidobacterium breveHH-BR26 hmoov yog 2.57 × 10¹¹CFU/g. Tom qab lub sijhawm cia ntawm 24 lub hlis, lub zog ntawm cov hmoov yog 1.38 × 10¹¹CFU/g. Nws txhais tau tias cov kab mob no tuaj yeem nyob ruaj khov rau lub sijhawm ntev yog tias khaws cia ntawm qhov kub thiab txias.

Fig 3. Cia stability ntawmB.breveHH-BR26

KEV PAB CUAM

1. Antioxidant muaj peev xwm

Hauv cov neeg noj qab haus huv, qhov sib npaug ntawm cov dawb radical ntau lawm thiab kev tiv thaiv antioxidant. Kev cuam tshuam ntawm qhov sib npaug no tuaj yeem ua rau muaj kab mob. Cov pa oxygen ntau dhau, kev ntxhov siab ntawm lub cev, kev kis mob, thiab kev raug mob yog txuam nrog ROS thiab RNS overproduction. Los ntawm kev sim colorimetric (Table 1), peb pom tias nyobB.breveHH-BR26 thiab nws cov cell fragments pom muaj zog DPPH scavenging kev ua amd tuaj yeem tiv thaiv lipid-peroxidation.

	DPPH		Tiv thaiv lipid-peroxidation
	A₅₁₇	kev tshem tawm tus nqi (%)	A₅₃₂	kev tshem tawm tus nqi (%)
Tswj	0.399±0.001	-	0.369±0.017	-
Cov kab mob nyob	0.357±0.009	10.542±3.851	0.243±0.009	34.073±0.082
Cell tawg	0.293±0.005	26.426±1.212	0.120±0.010	67.581±1.142