Ua pa phem Relief Probiotics

1. Khoom taw qhia

Khoom npe: Oral tsw probiotic hmoov
Khoom specifications: 2g * 30 daim / lub thawv
Cov Lus Qhia: Cov kab mob sib xyaw ua ke muab los ntawm cov khoom no tuaj yeem cuam tshuam kev loj hlob ntawm cov kab mob tseem ceeb uas ua rau ua pa tsis zoo, thiab tuaj yeem txo cov tsos mob ua pa phem.
Cov lus qhia rau kev siv: 3 zaug hauv ib hnub, 1 ntsiav tshuaj txhua zaus; nws raug pom zoo kom noj cov hmoov qhuav ntawm qhov ncauj ncaj qha tom qab noj mov. Cov neeg uas ua tsis taus pa ntev yuav tsum tau noj nws ntev mus txog thaum lawv ua pa tshiab.
Txoj kev khaws cia: khaws cia rau hauv qhov chaw txias, nyiam dua lub tub yees ntawm 4 degree.
Cov suav suav tau: Probiotic sib xyaw (50 billion CFU/g), Bacillus coagulans PB-BC 02 (20 billion CFU/g) thiab Lactobacillus salivarius HH-LS 17 (30 billion CFU/g).
Lwm cov khoom xyaw: 200mg sorbitol, 200mg citric acid, 200mg peppermint hmoov, 200mg vitamin C, 100mg fructooligosaccharides, 200mg galactooligosaccharides, 100mg sucralose, 100mg zaub mov tsw.

2. Pathogenesis

Qhov ncauj flora yog ib qho tseem ceeb ntawm lub qhov ncauj mucosal barrier thiab tiv thaiv kab mob, uas tuaj yeem tiv thaiv kab mob ntxeem tau thiab tiv thaiv thiab kho kab mob ntawm qhov ncauj. Qhov ncauj microecological imbalance tuaj yeem rhuav tshem lub qhov ncauj mucosal barrier thiab muab tej yam kev mob rau lub colonization ntawm tsw tsim cov kab mob, yog li ua rau lub qhov ncauj kab mob xws li halitosis. Qhov ncauj halitosis (IOH) suav txog 80% mus rau 90% ntawm halitosis thiab feem ntau yog tshwm sim los ntawm kev tsim cov tshuaj sulfur tsis haum los ntawm cov kab mob hauv qhov ncauj. Fusobacterium nucleatum tuaj yeem decompose sulfur-muaj substrates nyob rau hauv lub qhov ncauj kab noj hniav mus rau volatile sulfur compounds. Volatile Sulfur Compounds (VSCs), thiab lawv cov metabolites tuaj yeem tsim cov tshuaj xws li skatole, cadaverine, indole, thiab putrescine, uas tuaj yeem ua rau qhov ncauj malodor.
Tam sim no, cov kev kho mob ua pa phem feem ntau suav nrog kev tshem tawm txhua yam (tshuaj hniav, nplaig scraping, thiab lwm yam), tshuaj kho mob (chlorhexidine qhov ncauj ntxuav, zom cov pos hniav, thiab lwm yam), thiab siv cov tshuaj suav tshuaj thiab cov khoom ntuj tsim (tshuaj yej polyphenols. , vitamin C, catechins, thiab lwm yam). thiab kev siv cov tshuaj probiotics, thiab lwm yam. Cov kev kho mob ib txwm muaj rau lub sij hawm luv luv, thiab kev siv mus sij hawm ntev tuaj yeem ua rau qhov ncauj flora tsis txaus. Probiotics tuaj yeem tswj hwm qhov ncauj ntawm cov hauv paus hniav thiab inhibit qhov kev loj hlob ntawm cov kab mob uas tsim cov ntxhiab tsw. Yog li ntawd, kev siv probiotics los kho halitosis tau maj mam dhau los ua qhov kev tshawb fawb hotspot.

3. Mechanism ntawm probiotics txhim kho pa phem

Zoo li cov kab mob uas muaj kab mob microbial, xws li cov hniav caries thiab periodontitis, ua pa phem tuaj yeem kho microbially los ntawm kev tsom cov kab mob ua rau muaj feem cuam tshuam nrog nws. Probiotics tuaj yeem cuam tshuam kev loj hlob ntawm cov kab mob hauv qhov ncauj, tswj cov kab mob hauv qhov ncauj, thiab tswj kev noj qab haus huv ntawm qhov ncauj microecology. Lub ntsiab mechanisms uas probiotics ua hauj lwm muaj xws li cov nram qab no yam: (1) Sib tw rau khi qhov chaw, txo cov colonization ntawm cov kab mob pathogenic, thiab sib sau ua ke nrog cov kab mob pathogenic. ; (2) Sib tw rau cov khoom noj khoom haus thiab kev loj hlob thiab tsim cov tshuaj tua kab mob; (3) Txhim kho tus tswv tsev lub cev tiv thaiv kab mob thiab inhibit zus tau tej cov pro-inflammatory cytokines induced los ntawm cov kab mob.

4. Hehe Biotech's probiotics los txhim kho kev ua pa tsis zoo

Tus naj npawb ntawm Bacillus coagulans yog PB-BC02 thiab lub npe yog Bacillus coagulans. Nws tau muab tso rau hauv Tuam Tshoj Hom Culture Collection Center ntawm Wuhan University hauv Wuhan, Suav. Hnub tso nyiaj yog Lub Kaum Ob Hlis 12, 2018. Tus lej tso nyiaj yog CCTCC NO: M2018889.

5. Kev ntsuam xyuas cov txiaj ntsig ntawm kev txhim kho ua pa phem

Hauv txoj kev tshawb no, 5 hom kab mob probiotic tau pib tshuaj ntsuam xyuas los ntawm kev ntsuam xyuas lub peev xwm ntawm probiotics los tiv thaiv kev tsim H2S los ntawm Fusobacterium nucleatum, thiab ntxiv kev soj ntsuam lub peev xwm ntawm 5 hom probiotics los tiv thaiv kev tsim cov tshuaj sulfides (VSCs) thiab biofilm los ntawm Fusobacterium. nucleatum. Bacillus coagulans PB-BC 02 thiab Lactobacillus salivarius HH-LS 17 yog ob hom probiotics uas muaj txiaj ntsig zoo hauv vitro. Thaum kawg, qhov inhibitory nqi ntawm ib leeg-strain probiotics thiab compound probiotics ntawm kev loj hlob ntawm Fusobacterium nucleatum tau muab piv. Cov txiaj ntsig tau pom tias qhov nyuaj Cov nyhuv inhibitory ntawm probiotics rau kev loj hlob ntawm Fusobacterium nucleatum yog qhov zoo dua li ntawm cov kab mob ib leeg, qhia tias kev sib xyaw ntawm Bacillus coagulans PB-BC 02 thiab Lactobacillus salivarius HH-LS 17 muaj cov txiaj ntsig ntawm kev txhim kho ua pa tsis zoo. . Cov txheej txheem xeem thiab cov txiaj ntsig yog raws li hauv qab no:

5.1 Lub peev xwm ntawm probiotics inhibit H2S ntau lawm los ntawm Fusobacterium nucleatum

Lub hauv paus ntsiab lus xeem: Cov tshuaj tiv thaiv ntawm H2S thiab FeSO4 los tsim cov dub ferrous sulfide FeS precipitate yog siv los ntsuas lub peev xwm ntawm cov probiotics sib txawv los inhibit H2S ntau lawm los ntawm Fusobacterium nucleatum.
Kev ua haujlwm tshwj xeeb: Ua kom muaj kev daws teeb meem ntawm ferrous sulfate thiab sodium thiosulfate, lim nws los ntawm {{0}}.22 μm sterile lim membrane, thiab ntxiv rau BHI kab lis kev cai nruab nrab kom ua tiav qhov kawg ntawm ferrous sulfate thiab sodium thiosulfate 0.2‰ thiab thiosulfate 0.3‰, Tom qab ntawd, 2% 109CFU/mL Fusobacterium nucleatum cov kab mob raug tshem tawm tau ntxiv, tom qab ntawd 5% cov kab mob probiotic tov, thiab kab mob anaerobically ntawm 37 degree rau 36 h.
Cov txiaj ntsig kev xeem: Kev tshuaj ntsuam raws li cov nag lossis daus tsim. "-" txhais tau hais tias tsis muaj nag lossis daus, qhia tias probiotics muaj zog inhibitory muaj peev xwm tiv thaiv Fusobacterium nucleatum. "+" muaj nag los me ntsis, "++" muaj nag lossis daus ntau, thiab " +++" Muaj cov av qeeg. Cov txiaj ntsig tau qhia hauv Table 1. Ntawm 31 hom kab mob probiotic, 11 hom muaj nag lossis daus me ntsis, 8 hom muaj nag lossis daus ntau, 7 hom muaj nag lossis daus loj, thiab 5 hom kab mob (Lactobacillus acidophilus HH-LA26, Lactobacillus paracase. HH-LP58, Lactobacillus salivarius HH-LS17, Lactobacillus bifidum HH-BL18 thiab Bacillus coagulans PB-BC 02) tsis pom muaj nag lossis daus, qhia tias lawv muaj peev xwm txhim kho halitosis.

Table 1 Muaj peev xwm ntawm 31 probiotic hom los inhibit H2S ntau lawm los ntawm Fusobacterium nucleatum

5.2 Lub peev xwm ntawm probiotics los tiv thaiv kev tsim cov tshuaj tsis haum tshuaj sulfur (VSCs) los ntawm Fusobacterium nucleatum

Txoj kev sim: Tsib hom kab mob probiotics tau pib tshuaj ntsuam xyuas raws li lawv lub peev xwm los inhibit H2S ntau lawm los ntawm Fusobacterium nucleatum, thiab VSCs uas tsim los ntawm Fusobacterium nucleatum tau txheeb xyuas ntxiv siv Halimeter ntsuas. Tag nrho ntawm 2 mL ntawm 107 CFU/mL Fusobacterium nucleatum kab mob ncua kev kawm ntawv thiab 15% (v/v) cov kab mob probiotic tov tau ntxiv rau lub raj tshuaj Hungate. Tom qab kev sib koom ua ke rau 9 teev, probiotics 'muaj peev xwm inhibit qhov tsim ntawm Fusobacterium nucleatum raug ntsuas. muaj peev xwm ntawm VSCs.
Cov txiaj ntsig kev xeem: VSCs yog qhov tseem ceeb ntawm kev ua pa phem. Lawv tsis tsuas yog cuam tshuam txog qib ntawm kev ua pa tsis zoo, tab sis kuj ua rau cov pos hniav puas, uas yog qhov qhia tau tseem ceeb heev. Los ntawm kev ntsuas tus nqi inhibitory ntawm tsib hom kab mob tau los ntawm kev tshuaj ntsuam ua ntej ntawm VSCs uas tsim los ntawm Fusobacterium nucleatum, cov kab mob tau kuaj ntxiv. Cov txiaj ntsig tau pom nyob rau hauv Table 2. Ntawm lawv, qhov muaj peev xwm inhibitory ntawm 3 hom yog ntau dua 80%, thiab qhov inhibitory tus nqi ntawm 2 hom yog ntau dua 80%. Hauv qab 70%. Yog li, peb hom kab mob probiotic suav nrog Lactobacillus salivarius HH-LS17, Lactobacillus bifidus longum HH-BL18, thiab Bacillus coagulans PB-BC02 raug xaiv los kawm txog lawv cov teebmeem ntawm kev tsim biofilm ntawm Fusobacterium nucleatum.

Table 2 Cov nyhuv ntawm probiotics ntawm VSCs ntau lawm los ntawm Fusobacterium nucleatum

5.3 Lub peev xwm ntawm probiotics inhibit Fusobacterium nucleatum biofilm tsim

Txoj kev sim: Kho cov kab mob siab ntawm Fusobacterium nucleatum mus rau 107 CFU/mL thiab cia nws sawv ntsug li 9 teev. Tom qab ntawd ntxiv nws mus rau 96- phaj dej. Ntxiv 180 μL ntawm Fusobacterium aggregates kab mob ncua thiab 20 μL ntawm cov kab mob probiotic tov rau txhua qhov dej. 37 Cultivation ntawm degree rau 48 h. Nyob rau hauv pawg tswj tsis zoo, tib lub ntim ntawm MRS nruab nrab tau ntxiv es tsis txhob ntawm cov kua probiotic, thiab nyob rau hauv cov kev tswj tsis pub muaj, tib lub ntim ntawm BHI tau ntxiv es tsis txhob ntawm cov kua probiotic. Tom qab incubation, ntxuav ob zaug nrog PBS tsis, ces kho nrog 99% methanol rau 15 min, muab pov tseg lub supernatant, thiab qhuav nyob rau hauv chav tsev kub. Tom qab ziab kom tiav, ntxiv 100 μL ntawm 0.1% crystal violet tov rau txhua qhov dej, stain rau 5 feeb, ntxuav ob zaug nrog dej tsis huv tom qab staining, thiab tom qab ntawd muab tso rau hauv chav tsev kub kom txog thaum qhuav tag. Thaum kawg, ntxiv 200 μL ntawm 33% acetic acid tov rau kev sib cais. Tom qab sib tov nrog ib tug pipette, pipet 175 μL los ntawm txhua lub qhov dej thiab ntxiv rau ib tug tshiab 96-zoo phaj, thiab nyeem cov absorbance tus nqi ntawm 570 nm nrog ib tug microplate nyeem ntawv. Txoj kev suav ntawm biofilm-mediated txo yog inhibition tus nqi (%)=(tsis zoo tswj biofilm - tus nqi ntawm supernatant intervention biofilm) / tsis zoo tswj biofilm.
Cov txiaj ntsig kev xeem: Periodontal hnab ris thiab nplaig txheej yog qhov muaj feem cuam tshuam rau kev ua pa tsis zoo vim tias lawv muab qhov chaw loj hlob zoo rau cov kab mob uas tsim VSCs thiab lwm yam ntxhiab tsw molecules. Cov kab mob feem ntau muaj nyob rau hauv daim ntawv ntawm biofilms nyob rau hauv cov hnab ris periodontal thiab nplaig txheej. Fusobacterium nucleatum ua haujlwm ua cov kab mob nruab nrab ntawm cov kab mob hauv kev tsim cov quav hniav. Kev tsim ntawm nws cov biofilm yog tus yuam sij ua rau ua pa phem. Yog li ntawd, inhibiting nws biofilm yog ib kauj ruam tseem ceeb hauv alleviating pa phem. Muaj peev xwm muab tau. Cov txheej txheem siv lead ua violet staining yog siv los txiav txim siab lub peev xwm ntawm Lactobacillus los tiv thaiv kev tsim ntawm biofilm los ntawm Fusobacterium nucleatum. Cov txiaj ntsig tau pom nyob rau hauv Table 3. Thaum ntxiv 5% cov tshuaj probiotic kab mob, Bacillus coagulans PB-BC 02 thiab Lactobacillus salivarius HH-LS 17 muaj qhov zoo tshaj plaws inhibition nyhuv, tshaj 35%. Raws li cov txiaj ntsig ntawm inhibiting zus tau tej cov VSCs thiab biofilms los ntawm Fusobacterium nucleatum, Bacillus coagulans PB-BC 02 thiab Lactobacillus salivarius HH-LS 17 muaj kev cuam tshuam zoo hauv vitro. Tom ntej no, lawv cov teebmeem ntawm kev loj hlob ntawm Fusobacterium nucleatum raug soj ntsuam.

Table 3 Inhibitory teebmeem ntawm probiotics ntawm Fusobacterium nucleatum biofilm

5.4 Cov txiaj ntsig ntawm probiotics rau kev loj hlob ntawm Fusobacterium nucleatum

Kev sim txoj kev: Ntxiv 190 μL ntawm 107 CFU/mL Fusobacterium nucleatum kab mob ncua mus rau txhua qhov dej ntawm 96-zoo phaj, tom qab ntawd ntxiv 10 μL ntawm cov kab mob probiotic, kab lis kev cai anaerobically ntawm 37 degree, thiab saib xyuas ntawm 600 nm txhua 3 teev. Ntsuas tus nqi absorbance thiab kos ib qho kev loj hlob nkhaus.
Cov txiaj ntsig kuaj: Ntau yam kab mob ntawm qhov ncauj yog tshwm sim los ntawm qhov tsis sib xws ntawm qhov ncauj flora. Ib qho tshuaj tua kab mob muaj peev xwm pab probiotics tswj lub qhov ncauj flora. Cov txiaj ntsig tau pom nyob rau hauv daim duab 1. Cov nyhuv inhibitory ntawm probiotics ntawm Fusobacterium nucleatum yog nyob rau hauv kev txiav txim ntawm compound probiotics > Lactobacillus salivarius HH-LS 17 > Bacillus coagulans PB-BC 02 > MRS pab pawg tswj nruab nrab, qhia tias ob hom probiotics yog Nws tuaj yeem cuam tshuam kev loj hlob ntawm Fusobacterium nucleatum, thiab kev sib xyaw ntawm ob hom kab mob muaj qhov cuam tshuam zoo dua ntawm Fusobacterium nucleatum.

Daim duab 1 Cov txiaj ntsig ntawm probiotics ntawm kev loj hlob ntawm Fusobacterium nucleatum

Hauv cov ntsiab lus, Bacillus coagulans PB-BC 02 thiab Lactobacillus salivarius HH-LS 17 tuaj yeem cuam tshuam kev tsim H2S, VSCs thiab biofilm los ntawm Fusobacterium nucleatum, thiab muaj peev xwm inhibit qhov kev loj hlob ntawm Fusobacterium nucleatum tom qab kev sib xyaw ua ke muaj zog dua li ntawm ib hom kab mob, qhia tias kev sib xyaw ntawm Bacillus coagulans PB-BC 02 thiab Lactobacillus salivarius HH-LS 17 tuaj yeem txhim kho ua pa phem.

cov ntaub ntawv:

[1] Karbalaei M, Keikha M, Kobyliak NM, Khatib Zadeh Z, Yousefi B, Eslami M. Alleviation of halitosis los ntawm kev siv probiotics thiab lawv cov txheej txheem tiv thaiv hauv qhov ncauj kab noj hniav. New Microbes Tus Kab Mob Tshiab. 2021 Peb 23:42:100887. doi: 10.1016/j.nmni.2021.100887. PIB: 34123388; PIB: PMC8173312.
[2] Penala S, Kalakonda B, Pathakota KR, Jayakumar A, Koppolu P, Lakshmi BV, Pandey R, Mishra A. Kev ua tau zoo ntawm kev siv hauv zos ntawm probiotics ua ib qho ntxiv rau scaling thiab hauv paus npaj nyob rau hauv mob periodontitis thiab halitosis: Ib tug randomized tswj. kev sim. J Res Pharm Pract. 2016 Apr-Jun; 5(2):86-93. doi: 10.4103/2279-042X.179568. PIB: 27162801; PIB: PMC4843589.
[3] Jiang Zhentao. Kev tshuaj xyuas ntawm lactobacilli uas inhibit Fusobacterium nucleatum thiab kev soj ntsuam ntawm lawv cov txiaj ntsig hauv kev txhim kho halitosis [D]. Jiangnan University, 2022. DOI: 10.27169/d.cnki.gwqgu.2022.000533.
[4] Huang Zhiqiang, Cheng Yongbo. Kev tsim kho thiab kev ntsuam xyuas ntawm tus qauv nas halitosis tshwm sim los ntawm qhov ncauj flora imbalance [J]. Kev Tshawb Fawb Tshuaj Hauv Qhov Ncauj, 2023, 39(08): 745-750. DOI: 10.13701/j. cnki.kqyxyj.2023.08.015.
[5] Yang Wenjie, Ye Wei. Nyob rau hauv vitro kawm txog cov tshuaj tua kab mob ntawm ob hom probiotics ntawm halitosis-ua rau cov kab mob [J]. Oral Medicine, 2015, 35(03):179-182.DOI:10.13591/j.cnki.kqyx .2015.03.005.

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